Chapter 40 Neisseria and Moraxella catarrhalis Objectives 1. Identify the clinical specimens or sources for the isolation of pathogenic Neisseria spp. 2. List the Neisseria species considered normal flora and the sites where they colonize the human body. 3. Explain the routes of transmission for the organisms discussed in this chapter; include the clinical relevance of asymptomatic carriers.…

A study was performed which aimed at testing the reliability of our routine diagnostic tests for identification of Moraxella (Branhamella) catarrhalis in clinical samples from the respiratory tract. A preliminary diagnosis of 122 isolates as Moraxella catarrhalis was obtained by using colony morphology and results of Gram stain and oxidase test as the sole diagnostic criteria.

As the tools for defining bacterial genus and species relatedness become more refined, bacterial nomenclature necessarily becomes more complex. There is provided by the present invention, a method for detecting the presence of Branhamella catarrhalis, said method comprising; depositing and fixing on an inert support a sample containing DNA fragments in substantially single stranded form, contacting said fixed single stranded genetic material with a labelled probe consisting of a fragment of B. catarrhalis chromosomal DNA, identified TABLE 1. Chronology ofdifferential biochemical tests developed for Neisseria spp. andB. catarrhalis Test Date Authors (reference) Acid production from carbohydrates in Rich basal medium 1905 Dunnand Gordon (108) Low-concn peptone medium 1983 Knappand Holmes (178) Buffered salts solution 1970 Catlin (61) Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase.

Branhamella catarrhalis biochemical tests

Used in conjunction with other tests, butyrate discs achieve this by demonstrating the enzymatic hydrolysis of bromo-chloro-indolyl butyrate which creates a blue reaction. 2011-01-27 · These test results presented a conundrum as to the actual identity of both the mucoid and dry colony types of isolates. Insofar as the mucoid isolates were concerned, the biochemical test results were consistent with Moraxella (Branhamella) catarrhalis. medium containing ammonium and acetate. Isolated from the human nasopharynx (the main habitat), inflammatory secretions of the middle ear and maxillary sinus, from.

All B. catarrhalis strains tested gave a positive fluorescence reaction within 5 min, while Neisseria spp. remained negative, even after 18 h of incubation. Branhamella catarrhalis.

A total of 74 clinical isolates of Branhamella catarrhalis were characterized with respect to their ampicillin, amoxicillin-clavulanate, cephalothin, cefaclor, erythromycin, tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole MICs and zones of inhibition. Disk diffusion tests were perfo …

- 1 Extraction Observe established precautions against microbiological hazards throughout. Google has not performed a legal analysis and makes no representation as to the US5712118A (en), 1998-01-27, Vaccine for branhamella catarrhalis.

The hockey puck test was applied to these M. catarrhalis colonies, in which a wooden stick is used to try to push the colonies across the plate. The M. catarrhalis colonies scored positively on this test, which means they could be slid across the plate.

Branhamella catarrhalis biochemical tests

catarrhalis Test Date Authors (reference) Acid production from carbohydrates in Rich basal medium 1905 Dunnand Gordon (108) Low-concn peptone medium 1983 Knappand Holmes (178) Buffered salts solution 1970 Catlin (61) Branhamella catarrhalis can be distinguished from Neisseria spp.

It Se hela listan på antimicrobe.org These gram-negative cocci resemble Neisseria species but can be readily distinguished by routine biochemical tests after culture isolation from infected fluids or tissues. All strains now produce beta-lactamase. shares some physiological and biochemical characteristics, it is important to have a screening test for differentiation of Branhamella catarrhalis(1,2).
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- 1 Extraction Observe established precautions against microbiological hazards throughout.

However only in the past decade or so its role as a disease causing Swabs (carriers) CSF Glucose broth, Bile broth, Automated Incubation x 37° C x overnight & s/c BA/CA Colonies Biochemical tests, slide agglutination tests Uniformly sensitive to penicillin. CPC, Cefotaxime, Ceftriaxone Stuart’s medium Divided in 3 parts 3rd centrifuge – deposit for Gram stain & supernatant – Ag detection 1st part 2nd 1: 1 mix with GB – Incubate 2021-03-22 · Moraxella catarrhalis is a gram-negative, aerobic, oxidase-positive diplococcus that was first described in 1896. The organism has also been known as Micrococcus catarrhalis, Neisseria catarrhalis, and Branhamella catarrhalis; currently, it is considered to belong to the subgenus Branhamella of the genus Moraxella.
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In contrast, true cocci, such as most Neisseria spp. and Moraxella (Branhamella) catarrhalis, with which these organisms may be confused, maintain their original cocci shape in the presence of penicillin. In addition, the organisms discussed in this chapter do not use glucose and most do not grow on MacConkey agar but will grow well on blood

Se hela listan på catalog.hardydiagnostics.com Tests used for the identification of Moraxella catarrhalis. Typically positive results with Moraxella catarrhalis : The oxidase test. The catalase test. Tributyrin hydrolysis. Typically negative results with Moraxella catarrhalis : Acid from glucose. Acid from maltose.